2026年，单细胞测序与空间组学技术持续突破，从单一维度的转录组分析迈向多组学整合与高分辨率空间解析。多项重磅研究发表于Nature、Science、Nature Methods等顶级期刊，标志着生命科学研究进入"空间多组学"新时代。
CHARM技术：首次实现单细胞四组学同步捕获
2026年4月，北京大学团队在Nature发表研究，开发了名为CHARM的单细胞四组学测序技术，首次实现在同一细胞内同步捕获基因组三维空间结构、染色质可及性、组蛋白修饰和转录组四类关键组学信息。该技术为系统解析基因表达调控逻辑提供了全新的整合性技术框架，从时间、空间和细胞类型等多个维度刻画了多层表观调控与基因表达之间的复杂关系。
Fullscope-seq：大视野单细胞分辨率全长空间转录组
2026年7月，临港实验室魏武团队在Nature Methods发表研究，建立了大视野单细胞分辨率全长空间转录组技术Fullscope-seq。该技术突破了传统空间转录组在视野范围和转录本全长检测上的限制，能够在大组织切片中实现单细胞分辨率的全长转录本检测，助力神经系统疾病机制研究。同一基因可通过选择不同转录起始位点、可变剪接方式及转录终止位点，产生结构与功能各异的转录本异构体，Fullscope-seq为解析这些异构体的空间分布提供了关键工具。
Cellist：高分辨率空间转录组精准跨平台单细胞分割
2026年5月，同济大学王晨飞/张鹏团队在Nature Genetics发表研究，开发了全新的多模态细胞分割方法Cellist。该方法突破传统思路，创新性提出以转录本精准归属为核心的细胞分割新范式，不追求完美还原细胞形态边界，而是聚焦于将每个转录本准确分配至其来源细胞，从而获取高纯度的单细胞表达谱。Cellist融合染色图像与空间转录组数据，实现了高分辨率空间转录组的精准跨平台单细胞分割。
Spatial Perturb-Seq：完整组织内的单细胞功能基因组学
2026年9月，研究团队在Nature Communications发表研究，开发了Spatial Perturb-Seq技术，一种体内CRISPR技术，能够在完整组织的单细胞中同时探究多个基因，并且兼容基于测序和基于探针的空间技术。研究人员将Spatial Perturb-Seq应用于小鼠大脑中神经发育相关基因的功能筛选，首次在完整组织结构内实现了高通量的单细胞功能基因组学研究，为理解基因功能在组织微环境中的作用提供了全新手段。
临床应用：三阴性乳腺癌化疗耐药全景图
2026年7月，MD Anderson团队在Nature发表研究，借助10x Genomics单细胞与Visium+Xenium空间多组学技术，对101例三阴性乳腺癌患者治疗前样本进行深度解析，从"细胞身份"到"空间布局"逐层揭示了化疗耐药的机制。研究发现了三阴性乳腺癌的不同生态型（Ecotypes），这些生态型与化疗响应密切相关，为精准治疗和耐药预测提供了新的生物标志物。
百翌博代理产品支持
武汉百翌博科技有限公司代理的多个品牌为单细胞与空间组学研究提供关键试剂与耗材支持。Cellecta提供CRISPR文库与慢病毒载体，适用于Spatial Perturb-Seq等功能基因组学筛选；Jena Bioscience提供核酸修饰试剂与分子生物学工具，支持单细胞多组学文库构建；ImmunoReagents提供高质量抗体试剂，可用于空间转录组的蛋白验证与免疫荧光共定位。公司承诺100%原装进口、官方授权可查、冷链运输保障，为科研工作者提供稳定可靠的试剂供应。
全国免费热线：4006-027-696 | 邮箱：info@bioinborn.com
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